Western Blotting (WB), ELISA, Immunofluorescence (IF)
Specificity
At least six alternatively spliced transcript variants have been observed.
Purification
CASK Antibody is affinity chromatography purified via peptide column.
Immunogen
CASK antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CASK. The immunogen is located within amino acids 760 - 810 of CASK.
CASK
Reactivity: Mouse, Rat, Hamster
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
CASK antibody can be used for detection of CASK by Western blot at 1 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
CASK Antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
CASK antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Target
CASK
(Calcium/calmodulin-Dependent serine Protein Kinase (MAGUK Family) (CASK))
CASK Antibody: CASK (Calcium/calmodulin-dependent serine protein kinase), a conserved multi-domain scaffolding protein, belongs to a MAGUK (membrane-associated guanylate kinase homologs) subfamily and is involved in cell junction organization, tumor suppression and signaling. It is characterized by a novel domain structure that consists of a calcium/calmodulin-dependent protein kinase domain followed by PDZ, SH3 and guanylate kinase-like (GUK) domains. CASK is ubiquitously expressed and significantly greater in brain where it is thought to be involved in signaling at neuronal synapses. CASK interacts with CASKINs and defects in CASK are the cause of mental retardation X-linked CASK-related.