Estrogen Receptor alpha antibody (AA 250-300)

Catalog No. ABIN6991887

The Rabbit Polyclonal anti-Estrogen Receptor alpha antibody (ABIN6991887) specifically detects Estrogen Receptor alpha in WB, ELISA, IF and IHC (p). The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for Estrogen Receptor alpha antibody (AA 250-300) (ABIN6991887)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 250-300

Specificity: ESR1 antibody is human, mouse and rat reactive.

Purification: ESR1 Antibody is affinity chromatography purified via peptide column.

Immunogen: ESR1 antibody was raised against an 18 amino acid peptide near the center of human ESR1. The immunogen is located within amino acids 250 - 300 of ESR1.

Isotype: IgG

Application Details

Application Notes: ESR1 antibody can be used for detection of ESR1 by Western blot at 1 - 2 μ,g/mL.

Antibody validated: Western Blot in rat samples, Immunohistochemistry in rat samples and Immunofluorescence in rat samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: ESR1 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: ESR1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ESR1

Background: ESR1 Antibody: Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors (1). Estrogen receptors, including ESR1, also known as ER-alpha and ESR2 (ER-beta), contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. ESR1 is a widely expressed nuclear protein and serves as a strong activator of estrogen responsive genes (1,2). Phosphorylation of serines 104 and 106, located in the N-terminal transcription activation function-1 domain (AF-1), plays a large role in regulating ER alpha activity (3).

Molecular Weight: Predicted: 65 kDa

Observed: 62 kDa

Gene ID: 2099

NCBI Accession: NP_001116214

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly