Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist.
Purification
P2RX7 antibody is affinity chromatography purified via peptide column.
Immunogen
P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7. The immunogen is located within the last 50 amino acids of P2RX7.
P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μ,g/mL. Antibody can also be used for immunocytochemistry starting at 5 μ,g/mL. For immunofluorescence start at 20 μ,g/mL.
Antibody validated: Western Blot in mouse samples, Immunocytochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Restrictions
For Research Use only
Format
Liquid
Concentration
1 mg/mL
Buffer
P2RX7 antibody is supplied in PBS containing 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
-20 °C,4 °C
Storage Comment
P2RX7 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Target
P2RX7
(Purinergic Receptor P2X, Ligand-Gated Ion Channel, 7 (P2RX7))
The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).