TIM3 antibody (N-Term)

Catalog No. ABIN6992198

Product Details anti-TIM3 Antibody

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Binding Specificity: AA 60-110, N-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TIM3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: At least two antibodies are known to exist, this antibody will detect both isoforms.

Purification: TIM-3 Antibody is affinity chromatography purified via peptide column.

Immunogen: TIM-3 antibody was raised against a peptide corresponding to 16 amino acids near the amino terminus of human TIM-3. The immunogen is located within amino acids 60 - 110 of TIM-3.

Isotype: IgG

Application Details

Application Notes: TIM-3 antibody can be used for Western blot at 1 - 2 μ,g/mL. Antibody can also be used for Immunohistochemistry at 2 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: TIM-3 Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: TIM-3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for TIM3

Target: TIM3 (TIM 3) (Hepatitis A Virus Cellular Receptor 2 (TIM 3))

Alternative Name: TIM-3 (TIM 3 Products)

Synonyms: HAVCR2 antibody, MGC140131 antibody, HAVcr-2 antibody, KIM-3 antibody, TIM3 antibody, TIMD-3 antibody, TIMD3 antibody, Tim-3 antibody, TIM-3 antibody, Tim3 antibody, Timd3 antibody, tim3 antibody, hepatitis A virus cellular receptor 2 antibody, HAVCR2 antibody, Havcr2 antibody

Target Type: Virus

Background: The immune checkpoint protein TIM-3 is a member of the immunoglobulin superfamily and TIM family of proteins that was initially identified as a specific marker of fully differentiated IFN-? producing CD4 T helper 1 (Th1) and CD8 cytotoxic cells. It is a Th1-specific cell surface protein that regulates macrophage activation and negatively regulates Th1-mediated auto- and alloimmune responses, and is also highly expressed on regulatory T cells, monocytes, macrophages, and dendritic cells (1). TIM-3 and PD-1 are co-expressed on most CD4 and CD8 T cells infiltrating solid tumors or in hematologic malignancy in mice, blocking TIM-3 in conjugation with a PD-1 blockade increases the functionality of exhausted T cells and synergizes with to inhibit tumor growth (2,3).

Molecular Weight: Predicted: 33 kDa

Observed: 37 kDa

Gene ID: 84868

NCBI Accession: NP_116171

UniProt: Q8TDQ0

Pathways: Regulation of Lipid Metabolism by PPARalpha, Cancer Immune Checkpoints