VISTA antibody (C-Term)

Catalog No. ABIN6992209

Product Details anti-VISTA Antibody

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Binding Specificity: C-Term

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF)

Purification: VISTA Antibody is affinity chromatography purified via peptide column.

Immunogen: VISTA antibody was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human VISTA. The immunogen is located within the last 60 amino acids of VISTA.

Isotype: IgG

Application Details

Application Notes: VISTA antibody can be used for Immunohistochemistry at 2 μ,g/mL. For Immunoflorescence start at 20 μ,g/mL. For Immunocytochemistry start at 1 μ,g/mL.

Antibody validated: Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VISTA Antibody is supplied in PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VISTA (VISTA Products)

Synonyms: MGC112715 antibody, MGC151567 antibody, B7-H5 antibody, B7H5 antibody, GI24 antibody, PP2135 antibody, SISP1 antibody, Dies1 antibody, VISTA antibody, V-set immunoregulatory receptor antibody, vsir antibody, Vsir antibody, VSIR antibody

Background: The lymphocyte activation gene-3 (LAG3) is a member of the immunoglobulin superfamily and binds MHC class II with high affinity (1), negatively regulating T-cell function and homeostasis (2). It is expressed in B, T, and NK cells, monocytes, and dendritic cells (3), and acts to regulate T cell expansion (4). LAG3 is also an important immune checkpoint protein, with anti-LAG3 antibodies activating T effector cells and affecting regulatory T cell functions. Furthermore LAG3 appears to act in a synergistic fashion with PD-1/PD-L1, suggesting that a dual antibody approach may prove useful in cancer immunotherapy (5).

Gene ID: 64115

NCBI Accession: NP_071436

UniProt: Q9H7M9

Pathways: Cancer Immune Checkpoints