VISTA antibody (Extracellular Domain)

Catalog No. ABIN6992256

Product Details anti-VISTA Antibody

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Binding Specificity: Extracellular Domain

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This VISTA antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Purification: VISTA Antibody is supplied as protein A purified IgG1.

Immunogen: VISTA antibody was raised against the extracellular domain of human VISTA.

Clone: 4C4

Isotype: IgG1

Application Details

Application Notes: VISTA antibody can be used for detection of VISTA by Western blot at 0.25 - 1 μ,g/mL. Antibody can also be used for immunohistochemistry starting at 2 μ,g/mL and immunocytochemistry starting at 1 μ,g/mL. For immunofluorescence start at 2 μ,g/mL.

Antibody validated: Western Blot in human samples, Immunohistochemistry in human samples, Immunocytochemistry in human samples, Immunofluorescence in human samples and Flow Cytometry in mouse samples. All other applications and species not yet tested.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: VISTA Antibody is supplied in PBS containing 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,4 °C

Storage Comment: VISTA antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Target Details for VISTA

Target: VISTA (V-type immunoglobulin domain-containing suppressor of T-cell activation (VISTA))

Alternative Name: VISTA (VISTA Products)

Synonyms: MGC112715 antibody, MGC151567 antibody, B7-H5 antibody, B7H5 antibody, GI24 antibody, PP2135 antibody, SISP1 antibody, Dies1 antibody, VISTA antibody, V-set immunoregulatory receptor antibody, vsir antibody, Vsir antibody, VSIR antibody

Background: VISTA Antibody: VISTA/B7-H5/platelet receptor Gi24 is a single-pass type I membrane protein located at the cell surface. It is an immunoregulatory receptor which can inhibit T-cell response and may promote differentiation of embryonic stem cells, by inhibiting the BMP4 signaling pathway. The protein can be cleaved by MMP14, and stimulate MMP14-mediated MMP2 activation.

Molecular Weight: Predicted: 31 kDa

Observed: 47 kDa

Gene ID: 64115

NCBI Accession: NP_071436

UniProt: Q9H7M9

Pathways: Cancer Immune Checkpoints