SARS-CoV-2 ORF3b (N-Term) antibody
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- Target
- SARS-CoV-2 ORF3b
- Binding Specificity
- N-Term
- Reactivity
- SARS Coronavirus-2 (SARS-CoV-2)
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- ELISA, Western Blotting (WB)
- Purification
- SARS-CoV-2 (COVID-19) ORF3b Antibody is affinity chromatography purified via peptide column.
- Immunogen
- Anti-SARS-CoV-2 (COVID-19) ORF3b antibody was raised against a peptide corresponding to 13 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF3b protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF3b protein.
- Isotype
- IgG
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- Application Notes
- WB: 0.25 μ,g/mL Antibody validated: SARS-CoV-2 (COVID-19) ORF3b antibody can detect 2 ng of free peptide at 1 μ,g/mL in ELISA. It can detect SARS-CoV-2 ORF3b recombinant protein by ELISA and WB. All other applications and species not yet tested.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 mg/mL
- Buffer
- SARS-CoV-2 (COVID-19) ORF3b antibody is supplied in PBS containing 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C,4 °C
- Storage Comment
- SARS-CoV-2 (COVID-19) ORF3b antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
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- Target
- SARS-CoV-2 ORF3b
- Target Type
- Viral Protein
- Background
- Coronavirus disease 2019 (COVID-19), formerly known as 2019-nCoV acute respiratory disease, is an infectious disease caused by SARS-CoV-2, a virus closely related to the SARS virus (1). The disease is the cause of the 2019-20 coronavirus outbreak (2). SARS-CoV-2 virus proteins include structural proteins, non-structural proteins and accessory factors. The structure of SARS-CoV-2 consists of the following: a spike protein (S), hemagglutinin-esterease dimer (HE), a membrane glycoprotein (M), an envelope protein (E) a nucleoclapid protein (N) and RNA. SARS-CoV-2 non-structural protein is ORF1ab that consists of 16 proteins (nsp1-nsp16), while accessory factors include ORF3a, ORF3b, ORF6, ORF7a, ORF7b, ORF3b, ORF9b, ORF9c and ORF10. ORF3b may play a role in interferon antiviral system evasion (3).
- Molecular Weight
- 17kD kDa
- Gene ID
- 43740569
- UniProt
- P0DTC6, P0DTC3
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