Leptin antibody (AA 22-167)

Catalog No. ABIN7428662

This Mouse Monoclonal antibody specifically detects Leptin in WB. It exhibits reactivity toward Human.

Quick Overview for Leptin antibody (AA 22-167) (ABIN7428662)

Target: Leptin (LEP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Leptin antibody is un-conjugated

Application: Western Blotting (WB)

Clone: C5

Product Details anti-Leptin Antibody

Binding Specificity: AA 22-167

Purpose: Monoclonal Antibody to Leptin (LEP)

Specificity: The antibody is a mouse monoclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Leptin (LEP) corresdonding to Val22~Cys167 with N-terminal His and GST Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for Leptin

Target: Leptin (LEP)

Alternative Name: Leptin

Background: OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein

UniProt: P41159

Pathways: JAK-STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process