GCKR antibody (AA 90-286)

Catalog No. ABIN7426658

This anti-GCKR antibody is a Mouse Monoclonal antibody detecting GCKR in WB, IHC, IP and ICC. Suitable for Rat.

Quick Overview for GCKR antibody (AA 90-286) (ABIN7426658)

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GCKR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Clone: 4#

Product Details anti-GCKR Antibody

Binding Specificity: AA 90-286

Purpose: Monoclonal Antibody to Glucokinase Regulatory Protein (GKRP)

Specificity: The antibody is a mouse monoclonal antibody raised against GKRP. It has been selected for its ability to recognize GKRP in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Mouse

Purification: Protein A + Protein G affinity chromatography

Immunogen: Recombinant Glucokinase Regulatory Protein (GKRP) corresdonding to Val90~Ala286 (Accession # Q07071) with N-terminal His Tag

Isotype: IgG1 kappa

Application Details

Application Notes: Western blotting: 0.5-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-30 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-30 μg/mL,Immunohistochemistry in paraffin section: 5-30 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GCKR

Target: GCKR (Glucokinase (Hexokinase 4) Regulator (GCKR))

Alternative Name: GKRP

Background: GCKR, Glucokinase(hexokinase 4)regulator

UniProt: Q07071

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus