E-cadherin antibody (AA 155-266)

Catalog No. ABIN7430414

The Rabbit Polyclonal anti-E-cadherin antibody is suitable to detect E-cadherin in samples from Rat. It has been validated for WB, IHC, IP and ICC.

Quick Overview for E-cadherin antibody (AA 155-266) (ABIN7430414)

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This E-cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-E-cadherin Antibody

Binding Specificity: AA 155-266

Purpose: Polyclonal Antibody to E-cadherin

Specificity: The antibody is a rabbit polyclonal antibody raised against E-cadherin. It has been selected for its ability to recognize E-cadherin in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant E-cadherin corresdonding to Arg155~Phe266 (Accession # Q9R0T4)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.78 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for E-cadherin

Target: E-cadherin (CDH1) (Cadherin 1, Type 1, E-Cadherin (Epithelial) (CDH1))

Alternative Name: E-cadherin

Background: CDHE, CD324, CDH1, Arc1, ECAD, CDH-E, E-CAD, LCAM, UVO, Cadherin 1 Type 1, Uvomorulin, E-Cadherin, Calcium-Dependent Adhesion Protein, Epithelial, Liver Cell Adhesion Molecule

UniProt: Q9R0T4

Pathways: WNT Signaling, Sensory Perception of Sound, Cell-Cell Junction Organization, Tube Formation