IGF1 antibody (AA 33-143) (APC)

Catalog No. ABIN7429811

This Guinea Pig Polyclonal antibody specifically detects IGF1 in WB, IHC, IP and ICC. It exhibits reactivity toward Rabbit.

Quick Overview for IGF1 antibody (AA 33-143) (APC) (ABIN7429811)

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Reactivity: Rabbit

Host: Guinea Pig

Clonality: Polyclonal

Conjugate: This IGF1 antibody is conjugated to APC

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-IGF1 Antibody (APC)

Binding Specificity: AA 33-143

Purpose: Polyclonal Antibody to Insulin Like Growth Factor 1 (IGF1)

Specificity: The antibody is a cavia polyclonal antibody raised against IGF1. It has been selected for its ability to recognize IGF1 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Insulin Like Growth Factor 1 (IGF1) corresdonding to Gly33~Lys143 with N-terminal His and GST Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for IGF1

Target: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Alternative Name: IGF1

Background: IGFI, IGF1A, IBP1, MGF, Somatomedin C, Mechano Growth Gactor

UniProt: Q95222

Pathways: RTK Signaling, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation