COL4A1 antibody (AA 1444-1669)
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- Target See all COL4A1 Antibodies
- COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
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Binding Specificity
- AA 1444-1669
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This COL4A1 antibody is un-conjugated
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Application
- ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- Purpose
- Polyclonal Antibody to Collagen Type IV Alpha 1 (COL4a1)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against COL4a1. It has been selected for its ability to recognize COL4a1 in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Mouse, Rat
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Collagen Type IV Alpha 1 (COL4a1) corresdonding to His1444~Thr1669 with N-terminal His Tag
- Isotype
- IgG
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- Application Notes
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Western blotting: 0.5-2 μg/mL
Immunohistochemistry: 5-20 μg/mL
Immunocytochemistry: 5-20 μg/mL
Optimal working dilutions must be determined by end user.
- Comment
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
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- Target
- COL4A1 (Collagen, Type IV, alpha 1 (COL4A1))
- Alternative Name
- Collagen Type IV Alpha 1 (COL4A1 Products)
- Synonyms
- col4a1 antibody, wu:fc02g11 antibody, HANAC antibody, ICH antibody, POREN1 antibody, arresten antibody, Bru antibody, Col4a-1 antibody, Del(8)44H antibody, Raw antibody, Svc antibody, collagen, type IV, alpha 5 (Alport syndrome) antibody, collagen type IV alpha 1 chain antibody, collagen, type IV, alpha 1 antibody, col4a5 antibody, COL4A1 antibody, Col4a1 antibody
- Background
- COL4-A1, Arresten, Collagen Alpha-1(IV)chain
- Pathways
- Skeletal Muscle Fiber Development, Growth Factor Binding
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