MAP3K1 antibody (AA 1307-1509)

Catalog No. ABIN7442936

The Rabbit Polyclonal anti-MAP3K1 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect MAP3K1 in samples from Human.

Quick Overview for MAP3K1 antibody (AA 1307-1509) (ABIN7442936)

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-MAP3K1 Antibody

Binding Specificity: AA 1307-1509

Purpose: Polyclonal Antibody to Mitogen Activated Protein Kinase Kinase Kinase 1 (MAP3K1)

Specificity: The antibody is a rabbit polyclonal antibody raised against MAP3K1. It has been selected for its ability to recognize MAP3K1 in immunohistochemical staining and western blotting.

Cross-Reactivity: Mouse

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Mitogen Activated Protein Kinase Kinase Kinase 1 (MAP3K1) corresdonding to Ile1307~Arg1509 (Accession # Q13233)

Isotype: IgG

Application Details

Application Notes: Western blotting: 1-5 μg/mL,Immunocytochemistry in formalin fixed cells: 5-20 μg/mL,Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL,Immunohistochemistry in paraffin section: 5-20 μg/mL,Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for MAP3K1

Target: MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))

Alternative Name: MAP3K1

Background: MAP3-K1, MAPKKK1, MEKK, MEKK1

UniProt: Q13233

Pathways: MAPK Signaling, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades