CDK4 antibody (AA 6-295)

Catalog No. ABIN7438718

The Rabbit Polyclonal anti-CDK4 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect CDK4 in samples from Mouse.

Quick Overview for CDK4 antibody (AA 6-295) (ABIN7438718)

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDK4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-CDK4 Antibody

Binding Specificity: AA 6-295

Purpose: Polyclonal Antibody to Cyclin Dependent Kinase 4 (CDK4)

Specificity: The antibody is a rabbit polyclonal antibody raised against CDK4. It has been selected for its ability to recognize CDK4 in immunohistochemical staining and western blotting.

Cross-Reactivity: Human, Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cyclin Dependent Kinase 4 (CDK4) corresdonding to Tyr6~Leu295 (Accession # P30285)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-2 μg/mL,Immunohistochemistry: 5-20 μg/mL,Immunocytochemistry: 5-20 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for CDK4

Target: CDK4 (Cyclin-Dependent Kinase 4 (CDK4))

Alternative Name: CDK4

Background: CMM3, PSK-J3, Cell division protein kinase 4

UniProt: P30285

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, Regulation of Cell Size