GRB2 antibody (AA 1-217)

Catalog No. ABIN7434974

The Rabbit Polyclonal anti-GRB2 antibody (ABIN7434974) specifically detects GRB2 in IF and ICC. The antibody is reactive with Human samples.

Quick Overview for GRB2 antibody (AA 1-217) (ABIN7434974)

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GRB2 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunocytochemistry (ICC)

Product Details anti-GRB2 Antibody

Binding Specificity: AA 1-217

Purpose: Polyclonal Antibody to Growth Factor Receptor Bound Protein 2 (Grb2)

Specificity: The antibody is a rabbit polyclonal antibody raised against Grb2. It has been selected for its ability to recognize Grb2 in immunohistochemical staining and western blotting.

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Growth Factor Receptor Bound Protein 2 (Grb2) corresdonding to Met1~Val217 (Accession # P62993)

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.5-2 μg/mL,1:180-720Immunohistochemistry: 5-20 μg/mL,1:18-72Immunocytochemistry: 5-20 μg/mL,1:18-72Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.36 mg/mL

Buffer: 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for GRB2

Target: GRB2 (Growth Factor Receptor-Bound Protein 2 (GRB2))

Alternative Name: Grb2

Background: ASH, NCKAP2, EGFRBP-GRB2, Grb3-3, Adapter protein GRB2, Protein Ash, SH2/SH3 adapter GRB2

UniProt: P62993

Pathways: RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Actin Filament Polymerization, Hepatitis C, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, EGFR Downregulation