p21 antibody (AA 1-164)

Catalog No. ABIN7439048

The Rabbit Polyclonal anti-p21 antibody has been validated for WB, IHC, IP and ICC. It is suitable to detect p21 in samples from Human.

Quick Overview for p21 antibody (AA 1-164) (ABIN7439048)

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This p21 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)

Product Details anti-p21 Antibody

Binding Specificity: AA 1-164

Purpose: Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 1A (CDKN1A)

Specificity: The antibody is a rabbit polyclonal antibody raised against CDKN1A. It has been selected for its ability to recognize CDKN1A in immunohistochemical staining and western blotting.

Cross-Reactivity: Rat

Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Immunogen: Recombinant Cyclin Dependent Kinase Inhibitor 1A (CDKN1A) corresdonding to Met1~Pro164 with N-terminal His Tag

Isotype: IgG

Application Details

Application Notes: Western blotting: 0.01-3 μg/mL,Immunohistochemistry: 5-30 μg/mL,Immunocytochemistry: 5-30 μg/mL,Optimal working dilutions must be determined by end user.

Comment:

The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Expiry Date: 24 months

Target Details for p21

Target: p21 (CDKN1A) (Cyclin-Dependent Kinase Inhibitor 1A (p21, Cip1) (CDKN1A))

Alternative Name: CDKN1A

Background: WAF1, p21, CIP1, p21WAF1, CAP20, CDKN1, MDA-6, SDI1, p21CIP1, CDK-Interacting Protein 1, Melanoma differentiation-associated protein 6

UniProt: P38936

Pathways: p53 Signaling, PI3K-Akt Signaling, Cell Division Cycle, AMPK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Hepatitis C, Synthesis of DNA, Autophagy