Inhibitory Subunit of NF-KappaB epsilon (AA 207-440) antibody
-
- Target
- Inhibitory Subunit of NF-KappaB epsilon
- Binding Specificity
- AA 207-440
- Reactivity
- Human
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Purpose
- Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Epsilon (IkBe)
- Specificity
- The antibody is a rabbit polyclonal antibody raised against IkBe. It has been selected for its ability to recognize IkBe in immunohistochemical staining and western blotting.
- Cross-Reactivity
- Pig
- Purification
- Antigen-specific affinity chromatography followed by Protein A affinity chromatography
- Immunogen
- Recombinant Inhibitory Subunit Of NF Kappa B Epsilon (IkBe) corresdonding to Arg207~Pro440 with N-terminal His Tag
- Isotype
- IgG
-
- Application Notes
-
Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.
- Comment
-
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- Expiry Date
- 24 months
-
- Target
- Inhibitory Subunit of NF-KappaB epsilon
- Alternative Name
- Inhibitory Subunit Of NF Kappa B Epsilon
- Synonyms
- IKBE antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody
- Background
- NFKBIE, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Epsilon
-