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Inhibitory Subunit of NF-KappaB epsilon (AA 207-440) antibody

Reactivity: Human WB, ELISA, ICC, IHC (fro), IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7439441
  • Target
    Inhibitory Subunit of NF-KappaB epsilon
    Binding Specificity
    AA 207-440
    Reactivity
    Human
    Host
    Rabbit
    Clonality
    Polyclonal
    Application
    Western Blotting (WB), ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Purpose
    Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Epsilon (IkBe)
    Specificity
    The antibody is a rabbit polyclonal antibody raised against IkBe. It has been selected for its ability to recognize IkBe in immunohistochemical staining and western blotting.
    Cross-Reactivity
    Pig
    Purification
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    Immunogen
    Recombinant Inhibitory Subunit Of NF Kappa B Epsilon (IkBe) corresdonding to Arg207~Pro440 with N-terminal His Tag
    Isotype
    IgG
  • Application Notes

    Western blotting: 1-5 μg/mL Immunocytochemistry in formalin fixed cells: 5-20 μg/mL Immunohistochemistry in formalin fixed frozen section: 5-20 μg/mL Immunohistochemistry in paraffin section: 5-20 μg/mL Enzyme-linked Immunosorbent Assay: 0.05-2 μg/mL Optimal working dilutions must be determined by end user.

    Comment

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    Restrictions
    For Research Use only
  • Format
    Liquid
    Buffer
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    Expiry Date
    24 months
  • Target
    Inhibitory Subunit of NF-KappaB epsilon
    Alternative Name
    Inhibitory Subunit Of NF Kappa B Epsilon
    Synonyms
    IKBE antibody, NFKB inhibitor epsilon antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon antibody, NFKBIE antibody, Nfkbie antibody
    Background
    NFKBIE, Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Epsilon
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