Caspase 8 antibody (Cleaved-Asp384)

Catalog No. ABIN7251016

This anti-Caspase 8 antibody is a Rabbit Polyclonal antibody detecting Caspase 8 in WB, ELISA, IF and IHC (p). Suitable for Human.

Quick Overview for Caspase 8 antibody (Cleaved-Asp384) (ABIN7251016)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Caspase 8 Antibody

Binding Specificity: Cleaved-Asp384

Characteristics: Polyclonal Antibody

Purification: Affinity purification

Immunogen: Synthesized peptide derived from the C-terminal region of human Caspase-8

Isotype: IgG

Application Details

Application Notes: WB 1:500-2000, IHC 1:50-300, IF 1:50-300

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % sodium azide, 0.5 % BSA and 50 % glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background: Most upstream protease of the activation cascade of caspases responsible for the TNFRSF6/FAS mediated and TNFRSF1A induced cell death. Binding to the adapter molecule FADD recruits it to either receptor. The resulting aggregate called death-inducing signaling complex (DISC) performs CASP8 proteolytic activation. The active dimeric enzyme is then liberated from the DISC and free to activate downstream apoptotic proteases. Proteolytic fragments of the N-terminal propeptide (termed CAP3, CAP5 and CAP6) are likely retained in the DISC. Cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. Cleaves ADPRT. Hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-AMC. Likely target for the cowpox virus CRMA death inhibitory protein. Isoform 5, isoform 6, isoform 7 and isoform 8 lack the catalytic site and may interfere with the pro-apoptotic activity of the complex.

Molecular Weight:

Observed_MW: 47+55 kDa

Calculated_MW: 55 kDa

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades