HLA-DMB antibody

Catalog No. ABIN7252477

The Rabbit Polyclonal anti-HLA-DMB antibody is suitable to detect HLA-DMB in samples from Human. It has been validated for WB, ELISA and IHC.

Quick Overview for HLA-DMB antibody (ABIN7252477)

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DMB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HLA-DMB Antibody

Characteristics: Polyclonal Antibody

Purification: Antigen affinity purification

Immunogen: Fusion protein of human HLA-DMB

Isotype: IgG

Application Details

Application Notes: WB 1:500-1:2000, IHC 1:30-1:150, ELISA 1:5000-1:10000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.38 mg/mL

Buffer: PBS with 0.05 % Sodium azide and 40 % Glycerol, pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Avoid freeze / thaw cycles.

Target Details for HLA-DMB

Target: HLA-DMB (Major Histocompatibility Complex, Class II, DM beta (HLA-DMB))

Alternative Name: HLA-DMB

Background: HLA-DMB (Major Histocompatibility Complex, Class II, DM Beta) is a Protein Coding gene. Diseases associated with HLA-DMB include Red-Green Color Blindness and Red Color Blindness. Among its related pathways are ICos-ICosL Pathway in T-Helper Cell and CTLA4 Signaling. GO annotations related to this gene include MHC class II protein complex binding. An important paralog of this gene is ENSG00000248993.HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Molecular Weight:

Observed_MW: Refer to figures

Calculated_MW: 29 kDa

UniProt: P28068

Pathways: Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response