GZMB antibody (FITC)

Catalog No. ABIN7013929

The Mouse Monoclonal anti-GZMB antibody has been validated for ICFC. It is suitable to detect GZMB in samples from Human and Non-Human Primate.

Quick Overview for GZMB antibody (FITC) (ABIN7013929)

Target: GZMB (Granzyme B (GZMB))

Reactivity: Human, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This GZMB antibody is conjugated to FITC

Application: Intracellular Flow Cytometry (ICFC)

Clone: CLB-GB11

Product Details anti-GZMB Antibody (FITC)

Purpose: Anti-Granzyme B FITC

Specificity: The mouse monoclonal antibody CLB-GB11 recognizes granzyme B, a 31 kDa serine protease expressed intracellularly in activated Tc cells and NK cells.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Human NK cell line YT-INDY-derived granzyme B

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests. Intracellular staining.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: Granzyme B

Background: Granzyme B,Granzyme B is a serine protease that is expressed in cytoplasmic granules of cytotoxic T lymphocytes and NK cells. Vectorial secretion of perforin and granzymes is responsible for their granule-mediated cytotoxicity. Granzyme B plays a pivotal role in the induction of apoptosis in the target cells by activation of caspases. Moreover, granzyme B was reported to cleave directly alpha-tubulin, leading to perturbation of microtubule networks during the induced cell death.,GZMB, HLP, CTLA1, SECT

Gene ID: 3002

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis