HTR2A antibody (Extracellular, N-Term)

Catalog No. ABIN7887151

The Rabbit Polyclonal anti-HTR2A antibody (ABIN7887151) specifically detects HTR2A in WB, IHC and FACS. The antibody is reactive with Rat samples.

Quick Overview for HTR2A antibody (Extracellular, N-Term) (ABIN7887151)

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HTR2A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-HTR2A Antibody

Binding Specificity: AA 40-52, Extracellular, N-Term

Purpose: A Rabbit Polyclonal Antibody to 5-Hydroxytryptamine Receptor 2A

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 9,13 amino acid residues identical

Characteristics: Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody is directed against an epitope of the rat 5-A receptor. Anti-5HT2A Receptor (HTR2A) (extracellular) Antibody (ABIN7042875, ABIN7045286 and ABIN7045287) can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is ideal for detecting the receptor in living cells. It has been designed to recognize rat 5-hydroxytryptamine receptor 2A from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen: (C)SEASNWTIDAENR, corresponding to amino acid residues 40-52 of rat 5-Hydroxytryptamine receptor 2A

Isotype: IgG

Application Details

Application Notes:

WB: 1:200

FC: 5 mg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:1200

IP: The optimal concentration should be determined by the user

Comment:

Negative Control: (ABIN7234591)

Blocking Peptide: (ABIN7234591)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Concentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for HTR2A

Target: HTR2A (5-Hydroxytryptamine (serotonin) Receptor 2A (HTR2A))

Alternative Name: HTR2A

Background:

Synonyms: 5-Hydroxytryptamine receptor 2A, Serotonin receptor 2A

Description: 5-HT is involved in a diverse array of physiological and biological processes. In the brain, 5-HT affects sleep, mood, appetite, anxiety, aggression, perception, pain, and cognition1. Signaling of 5-HT is mediated by receptors that are located on the cell membrane of neurons and most other cells in the body. The 5-HT2 family consists of three G-protein coupled receptors (GPCRs): 5-HT2A, 5-HT2B, and 5-HT2C2. They are transmembrane proteins consisting of seven membrane-spanning α-helical segments with an extracellular N-terminus and an intracellular C-terminus. The binding of 5-HT to one of its receptors is thought to elicit a conformational change that activates associated heterotrimeric G proteins and recruits other downstream signaling/scaffolding molecules, such as GPCR kinases and β-arrestins.5-HT2A receptors have been found in many regions of the human brain including the cortex, brainstem, olfactory bulb, limbic system and basal ganglia3.The 5-HT2A receptor has been implicated in many mental disorders including schizophrenia, depression, obsessive compulsive disorder (OCD), attention deficit-hyperactivity disorder (ADHD), and eating disorders such as anorexia nervosa and autism spectrum disorders4.

Gene ID: 29595

NCBI Accession: NM_000621

UniProt: P14842

Pathways: JAK-STAT Signaling, Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process