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MICU1 antibody (Intracellular)

MICU1 Reactivity: Rat WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN7043334
  • Target See all MICU1 Antibodies
    MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))
    Binding Specificity
    • 7
    • 5
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 313-327, Intracellular
    Reactivity
    • 32
    • 8
    • 8
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Rat
    Host
    • 24
    • 7
    • 1
    Rabbit
    Clonality
    • 26
    • 6
    Polyclonal
    Conjugate
    • 15
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This MICU1 antibody is un-conjugated
    Application
    • 21
    • 14
    • 5
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB)
    Purpose
    A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)
    Specificity
    Intracellular
    Cross-Reactivity
    Mouse, Rat
    Predicted Reactivity
    Mouse - identical, human - 14,15 amino acid residues identical
    Characteristics
    Anti-MICU1 Antibody (ABIN7043334, ABIN7044111 and ABIN7044112) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.
    Purification
    Affinity purified on immobilized antigen.
    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1

    Isotype
    IgG
    Top Product
    Discover our top product MICU1 Primary Antibody
  • Application Notes

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

    Application Dilutions Western blot wb: 1:200

    Comment

    Negative Control: (ABIN7235963)

    Blocking Peptide: (ABIN7235963)

    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
    Concentration
    1 mg/mL
    Buffer
    PBS pH 7.4
    Storage
    4 °C,-20 °C
    Storage Comment

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target
    MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))
    Alternative Name
    MICU1 (MICU1 Products)
    Background
    Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS,Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.

    Alternative names: MICU1, Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS
    Gene ID
    365567
    NCBI Accession
    NM_006077
    UniProt
    Q6P6Q9
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