MICU1 antibody (Intracellular)

Catalog No. ABIN7043334

Product Details anti-MICU1 Antibody

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Binding Specificity: AA 313-327, Intracellular

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MICU1 antibody is un-conjugated

Application: Western Blotting (WB)

Purpose: A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)

Specificity: Intracellular

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: Mouse - identical, human - 14,15 amino acid residues identical

Characteristics: Anti-MICU1 Antibody (ABIN7043334, ABIN7044111 and ABIN7044112) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.

Purification: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1

Isotype: IgG

Application Details

Application Notes:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Comment:

Negative Control: (ABIN7235963)

Blocking Peptide: (ABIN7235963)

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4

Storage: 4 °C,-20 °C

Storage Comment:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Target Details for MICU1

Target: MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))

Alternative Name: MICU1 (MICU1 Products)

Background: Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS,Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.

Alternative names: MICU1, Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS

Gene ID: 365567

NCBI Accession: NM_006077

UniProt: Q6P6Q9