MICU1 antibody (Intracellular)
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- Target See all MICU1 Antibodies
- MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))
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Binding Specificity
- AA 313-327, Intracellular
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Reactivity
- Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This MICU1 antibody is un-conjugated
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Application
- Western Blotting (WB)
- Purpose
- A Rabbit Polyclonal Antibody to Mitochondrial Calcium Uptake Protein 1 (CBARA1)
- Specificity
- Intracellular
- Cross-Reactivity
- Mouse, Rat
- Predicted Reactivity
- Mouse - identical, human - 14,15 amino acid residues identical
- Characteristics
- Anti-MICU1 Antibody (ABIN7043334, ABIN7044111 and ABIN7044112) is a highly specific antibody directed against an epitope of the rat Mitochondrial calcium uptake protein 1 (CBARA1). The antibody can be used in western blot analysis. It has been designed to recognize CBARA1 from rat, mouse, and human samples.
- Purification
- Affinity purified on immobilized antigen.
- Immunogen
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Immunogen: Synthetic peptide
Immunogen Sequence: (C)EFERHDPVDGRISER, corresponding to amino acid residues 313-327 of rat MICU1
- Isotype
- IgG
- Top Product
- Discover our top product MICU1 Primary Antibody
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- Application Notes
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Antigen preadsorption control: 1 μg peptide per 1 μg antibody
Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A
Application Dilutions Western blot wb: 1:200
- Comment
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Negative Control: (ABIN7235963)
Blocking Peptide: (ABIN7235963)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- PBS pH 7.4
- Storage
- 4 °C,-20 °C
- Storage Comment
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Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.
Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).
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- Target
- MICU1 (Mitochondrial Calcium Uptake 1 (MICU1))
- Alternative Name
- MICU1 (MICU1 Products)
- Background
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Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS,Mitochondrial Ca2+ uptake was found to be mediated by the mitochondrial Ca2+ uniporter (MCU). MCU is a ruthenium-red-sensitive and highly selective Ca2+ ion channel that uptakes massive amount of Ca2+ across the inner membrane1. MICU1 is a ~54 kDa. protein with an amino-terminal mitochondrial targeting sequence, a predicted transmembrane helix, and a cytosolic C-terminus containing two classical EF-hand Ca2+-binding domains2.Mitochondrial calcium uptake 1 (MICU1) protein was identified as an essential element of mitochondrial Ca2+ uptake. MCU initially takes up Ca2+ very rapidly, but when [Ca2+] inevitably increases inside the matrix, MICU1 binds Ca2+ through its EF-hand domains, exerting an inhibitory role on MCU-dependent Ca2+ entry3.RNA expression of MICU1 is strongly correlated across a variety of tissues including: stomach, kidney, small and large intestine, cerebral cortex, cerebellum, hypothalamus, spinal cord and skeletal muscles4.Loss-of-function mutations in MICU1 causes a brain and muscle disorder linked to primary alterations in mitochondrial Ca2+ signaling5.
Alternative names: MICU1, Mitochondrial calcium uptake protein 1, Calcium-binding atopy-related autoantigen 1 homolog, ara CALC, CBARA1, MPXPS - Gene ID
- 365567
- NCBI Accession
- NM_006077
- UniProt
- Q6P6Q9
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