NGFR antibody

Catalog No. ABIN7044625

The Mouse Monoclonal anti-NGFR antibody is suitable to detect NGFR in samples from Rat. It has been validated for WB, IHC, IF and FACS.

Quick Overview for NGFR antibody (ABIN7044625)

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This NGFR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: 192-IgG

Product Details anti-NGFR Antibody

Purpose: A Mouse Monoclonal Antibody to Rat Neurotrophin Receptor p75NTR

Cross-Reactivity (Details): 192-IgG reacts with rat p75NTR. It does not cross-react with p75NTR of human or mouse origin.

Predicted Reactivity: Recognizes p75NTR from rat samples only

Characteristics: Mouse Anti-Rat p75 NGF Receptor (extracellular) Antibody (ABIN7043342, ABIN7044624, ABIN7044625 and ABIN7044626) is a highly specific monoclonal antibody directed against p75NTR. The antibody can be used in western blot, immunohistochemistry, and indirect flow cytometry applications. It has been designed to recognize p75NTR from rat samples only.

Purification: Affinity purified from cultured hybridoma medium.

Immunogen: Solubilized proteins from PC12 membranes.

Isotype: IgG1

Application Details

Application Notes:

WB: 1:150-1:2500

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:400

IP: The optimal concentration should be determined by the user

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 10 μL, 50 μL, 100 μL or 250 μL double distilled water (DDW), depending on the sample size.

Concentration: 1 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Target Details for NGFR

Target: NGFR (Nerve Growth Factor Receptor (NGFR))

Alternative Name: NGFR

Background:

Synonyms: p75NTR, NGFR p75, TNFR superfamily member 16, TNFRSF16, CD271

Description: p75NTR known as a cell surface receptor for NGF, BDNF, NT-3 and NT-4, is also a receptor for b-amyloid, and for the myelin axon repellant protein MAG.1,2,4,6 Binding of neurotrophins induces receptor dimerization followed by phosphorylation of receptor kinase residues and recruitment of intracellular proteins involved in signal transduction. p75NTR functions alone, or in functional complexes with the neurotrophin receptors trkA, trkB and trkC. p75NTR signals via interaction with different transducers such as TRAF6, IRAK, and RIP-2, activating NF-κB, JNK and p38, and inactivating rhoA.1,3,6 Receptor degradation is mediated by a metalloproteinase-dependent shedding of the extracellular domain. p75NTR binds all neurotrophins with similar nM affinity, but binds pro-neurotrophins with greater affinity, and is more potently activated by pro-neurotrophins.5Overexpression of the intracellular domain in developing neurons induces apoptosis. NGF-dependent association of NRIF with p75NTR contributes to pro-apoptotic signaling.7,8 Beta-amyloid peptide binds p75NTR and stimulates pro apoptotic signaling by p75NTR, suggesting that the receptor may be relevant for Alzheimer's disease.9

Gene ID: 24596

NCBI Accession: NM_002507

UniProt: Q08201

Pathways: NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding