Recombinant CCR5 antibody

Catalog No. ABIN7072654

This Mouse Monoclonal antibody specifically detects CCR5 in WB, ELISA, FACS, IF and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant CCR5 antibody (ABIN7072654)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: T21-8

Product Details anti-CCR5 Antibody

Purpose: Anti-CCR5 [T21/8], Mouse IgG2a, kappa

Specificity: T21/8 antibody binds with high affinity to a CCR5 N-terminal epitope. CCR5 is a receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunizing mice with intact RBL-CCR5 cells.

Isotype: IgG2a kappa

Application Details

Application Notes: T21/8 antibody is recommedned for the detection and analysis of CCR5 via various methods. For instance, one group used this antibody to detect intracellular localization of CCR5 in RBL-CCR5 cells via immunofluorescence staining (Pollok-Kopp et al., 2003). Another group investigated ligand-mediated down-modulation of CCR5 via flow cytometry, utilizing T21/8 antibody (Fox et al., 2015). With other anti-CCR5 antibodies which react with CCR5 phosphorylated on different serine residues (Ab01578, Ab01579), it can be used to study dynamic regulation of CCR5 and phosphorylation mechanisms.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background: CD195, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size