Recombinant CD40 antibody (Extracellular Domain)

Catalog No. ABIN7072685

The Rabbit Chimeric anti-CD40 antibody has been validated for BR. It is suitable to detect CD40 in samples from Human.

Quick Overview for Recombinant CD40 antibody (Extracellular Domain) (ABIN7072685)

Target: CD40

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD40 antibody is un-conjugated

Application: Blocking Reagent (BR)

Clone: Chi220

Product Details anti-CD40 Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD40 [chi220], Rabbit IgG, kappa

Specificity: This antibody binds the CD40 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG2a

Purification: Protein A affinity purified

Immunogen: This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody.

Isotype: IgG kappa

Application Details

Application Notes: This antibody blocks the interaction between CD40 and gp39. Chi220 was used either alone or in combination with CTLA4-Ig in an immunosuppresive therapy study to check the survival of renal allografts in non human primate models (PMID: 12394833). Chi220 blocks binding of CD154, it also has partial agonist properties and weak stimulatory potential. It synergisticaly works with LEA29Y (belatacept) to prolong the islet allograft survival in a non-human primate Macaca fascicularis (PMID: 15611281). A study on protection of islets xenographs in diabetic macaques used a combination of Chi220 with IL-2 receptor specific antibody Basiliximab for immunosuppression with induction therapy. Chi220 is used as an sCD154 blocking agent and it was seen that it binds sCD154 in a dose dependant manner (PMID: 21920020). Two humanized versions of this antibody were generated F4 and L3.17, also showed similar properties. Humanized versions of this antibody are available on request.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2a format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD40

Target: CD40

Alternative Name: CD40

Background: TNFSF5, CDw40, TRAP, p50, Bp50, CD40L receptor, Tumor necrosis factor ligand superfamily member 5, B-cell surface antigen CD40, T-cell antigen Gp39, TNF-related activation protein.

UniProt: P29965

Pathways: NF-kappaB Signaling, Cellular Response to Molecule of Bacterial Origin, M Phase, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints