CD47 antibody (AA 19-141, AA 198-207, AA 257-268) (Biotin)

Catalog No. ABIN7092998

The Biotin-conjugated Rabbit Monoclonal anti-CD47 antibody (Clone DM49) (ABIN7092998) specifically detects CD47 in FACS and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD47 antibody (AA 19-141, AA 198-207, AA 257-268) (Biotin) (ABIN7092998)

Target: CD47

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD47 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), ELISA

Clone: DM49

Product Details anti-CD47 Antibody (Biotin)

Binding Specificity: AA 19-141, AA 198-207, AA 257-268

Purpose: Biotinylated Anti-CD47 antibody(DM49), Rabbit mAb

Purification: Purified from cell culture supernatant by affinity chromatography

Immunogen: Recombinant human  CD47(Gln19-Glu141,Val198-Ala207,Ser257-Gly268) (ABIN6961081) produced by using human HEK293 cells

Isotype: IgG

Application Details

Application Notes: ELISA 1:5000-10000, Flow Cyt 1:100

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details for CD47

Target: CD47

Alternative Name: CD47

Background: Synonyms:CD47, MER6, IAP, OA3
Description:Leukocyte surface antigen CD47 is also known as Antigenic surface determinant protein OA3, Integrin-associated protein (IAP) and Protein MER6. CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain. CD47 is very broadly distributed on normal adult tissues. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins and plays an important role in memory formation and synaptic plasticity in the hippocampus by similarity. CD47 is the receptor for SIRPA, binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 Interaction with SIRPG mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation.

Molecular Weight: 35 kDa

Gene ID: 961

UniProt: Q08722