BrdU antibody

Catalog No. ABIN7112073

Product Details anti-BrdU Antibody

Target: BrdU (Bromodeoxyuridine (BrdU))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This BrdU antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: compound

Clone: 9E8

Isotype: IgG2a

Application Details

Application Notes: IHC: 1:200-1:1000, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for BrdU

Target: BrdU (Bromodeoxyuridine (BrdU))

Alternative Name: BrdU (BrdU Products)

Target Type: Chemical

Background: Synonyms:5-bromo-2-deoxyuridine, BrDu, BrdUrd, Bromodeoxyuridine, BUdR Background:Bromodeoxyuridine(5-bromo-2'-deoxyuridine, BrdU, BUdR, BrdUrd) is a synthetic nucleoside that is an analog of thymidine. It can be incorporated into the newly synthesized DNA of replicating cells(during the S phase of the cell cycle), substituting for thymidine during DNA replication. As such, BrdU is used for birth dating and monitoring cell proliferation. BrdU is a toxic and mutagenic substance. It triggers cell death, the formation of teratomas, alters DNA stability, lengthens the cell cycle, and has mitogenic, transcriptional and translational effects on cells that incorporate it. This antibody does not cross react with Thymidine.