Caspase 8 antibody (AA 1-270)

Catalog No. ABIN7112367

This anti-Caspase 8 antibody is a Mouse Monoclonal antibody detecting Caspase 8 in WB, IHC, ELISA, IP and IF. Suitable for Human.

Quick Overview for Caspase 8 antibody (AA 1-270) (ABIN7112367)

Target: Caspase 8 (CASP8)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Caspase 8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 2C2

Product Details anti-Caspase 8 Antibody

Binding Specificity: AA 1-270

Purpose: CASP8 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: 1-270aa

Immunogen: caspase 8, apoptosis-related cysteine peptidase

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000, IP: 1:200-1:2000, IHC: 1:100-1:400, IF: 1:50-1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for Caspase 8

Target: Caspase 8 (CASP8)

Alternative Name: CASP8

Background:

Synonyms: Caspase-8 (CASP-8)|Apoptotic cysteine protease|Apoptotic protease Mch-5|CAP4|FADD-homologous ICE/ced-3-like protease|FADD-like ICE (FLICE)|ICE-like apoptotic protease 5|MORT1-associated ced-3 homolog (MACH)|Caspase-8 subunit p18|Caspase-8 subunit p10|CASP8|MCH5

Background: Caspase 8, also named as MCH5, MACH, FLICE, and CAP4, belongs to the peptidase C14A family. It may participate in the GZMB apoptotic pathways and functions as an upstream regulator in a-bisabolol-induced apoptosis. Caspase 8 catalyzes an essential intermediate step in the ubiquitination and proteasome-mediated degradation of IRF3(PMID:21816816). It may control diabetic embryopathy-associated apoptosis via Caspase 8 is expressed as nine isoforms by alternative splicing with the molecular mass from 26 kDa to 62 kDa. This antibody can recognize pro-and cleaved-caspase 8.

Molecular Weight: 50-55 kDa, 25-32 kDa

Gene ID: 841

UniProt: Q14790

Pathways: Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Activation of Innate immune Response, Tube Formation, Positive Regulation of Endopeptidase Activity, Toll-Like Receptors Cascades