ERK1 antibody

Catalog No. ABIN7116410

The Mouse Monoclonal anti-ERK1 antibody is suitable to detect ERK1 in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for ERK1 antibody (ABIN7116410)

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This ERK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 8C11

Product Details anti-ERK1 Antibody

Purpose: ERK1/2 antibody

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen:

Immunogen sequence: peptide

Immunogen: mitogen-activated protein kinase 3

Isotype: IgG1

Application Details

Application Notes: WB:1:500-1:5000, IHC: 1:100-1:400, IF: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for ERK1

Target: ERK1 (MAPK3) (Mitogen-Activated Protein Kinase 3 (MAPK3))

Alternative Name: ERK1

Background:

Synonyms: Mitogen-activated protein kinase 3 (MAP kinase 3, MAPK 3)|ERT2|Extracellular signal-regulated kinase 1 (ERK-1)|Insulin-stimulated MAP2 kinase|MAP kinase isoform p44 (p44-MAPK)|Microtubule-associated protein 2 kinase|p44-ERK1|MAPK3|ERK1|PRKM3

Background: ERK1 and ERK2 belongs to the protein kinase superfamily. It is involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. ERK1/2 catalized the reaction: ATP + a protein = ADP + a phosphoprotein. It is activated by tyrosine phosphorylation in response to insulin and NGF.

Molecular Weight: 38-44 kDa

Gene ID: 5595

UniProt: P27361

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Growth Hormone Stimulus, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Hepatitis C, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGFR1 Specific Signals, S100 Proteins