RPA3 antibody

Catalog No. ABIN7118404

Product Details anti-RPA3 Antibody

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPA3 antibody is un-conjugated

Application: ELISA

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: replication protein A3, 14kDa

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for RPA3

Target: RPA3 (Replication Protein A3, 14kDa (RPA3))

Alternative Name: RPA3 (RPA3 Products)

Synonyms: REPA3 antibody, 14kDa antibody, C330026P08Rik antibody, im:6908405 antibody, zgc:56310 antibody, zgc:86697 antibody, rpa3 antibody, MGC82610 antibody, RPA3 antibody, repa3 antibody, replication protein A3 antibody, replication protein A3 S homeolog antibody, replication protein A3 L homeolog antibody, RPA3 antibody, Rpa3 antibody, rpa3 antibody, rpa3.S antibody, rpa3.L antibody

Background: Synonyms:REPA3, RPA14 Background:As part of the heterotrimeric replication protein A complex(RPA/RP-A), binds and stabilizes single-stranded DNA intermediates that form during DNA replication or upon DNA stress. It prevents their reannealing and in parallel, recruits and activates different proteins and complexes involved in DNA metabolism. Thereby, it plays an essential role both in DNA replication and the cellular response to DNA damage(PubMed:9430682). In the cellular response to DNA damage, the RPA complex controls DNA repair and DNA damage checkpoint activation. Through recruitment of ATRIP activates the ATR kinase a master regulator of the DNA damage response(PubMed:24332808). It is required for the recruitment of the DNA double-strand break repair factors RAD51 and RAD52 to chromatin, in response to DNA damage. Also recruits to sites of DNA damage proteins like XPA and XPG that are involved in nucleotide excision repair and is required for this mechanism of DNA repair(PubMed:7697716). Plays also a role in base excision repair(BER), probably through interaction with UNG(PubMed:9765279). Through RFWD3 may activate CHEK1 and play a role in replication checkpoint control. Also recruits SMARCAL1/HARP, which is involved in replication fork restart, to sites of DNA damage. May also play a role in telomere maintenance. RPA3 has its own single-stranded DNA-binding activity and may be responsible for polarity of the binding of the complex to DNA(PubMed:19010961). As part of the alternative replication protein A complex, aRPA, binds single-stranded DNA and probably plays a role in DNA repair. Compared to the RPA2-containing, canonical RPA complex, may not support chromosomal DNA replication and cell cycle progression through S-phase. The aRPA may not promote efficient priming by DNA polymerase alpha but could support DNA synthesis by polymerase delta in presence of PCNA and replication factor C(RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange(PubMed:19996105).

Gene ID: 6119

UniProt: P35244

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA