TDG antibody

Catalog No. ABIN7119947

The Rabbit Polyclonal anti-TDG antibody is suitable to detect TDG in samples from Human, Mouse and Rat. It has been validated for WB, ELISA and IP.

Quick Overview for TDG antibody (ABIN7119947)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP)

Product Details anti-TDG Antibody

Purpose: TDG antibody

Purification: Immunogen affinity purified

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: thymine-DNA glycosylase

Isotype: IgG

Application Details

Application Notes: WB: 1:500-1:2000, IP: 1:200-1:2000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze / thaw cycles.

Storage: -20 °C

Storage Comment: -20°C for 12 months

Expiry Date: 12 months

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background:

Synonyms: G/T mismatch-specific thymine DNA glycosylase|Thymine-DNA glycosylase (hTDG)|TDG

Background: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine(5fC) and 5-carboxylcytosine(5caC) in the context of CpG sites and mediates their excision through base-excision repair(BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine(5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil(5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Molecular Weight: 55-60 kDa

Gene ID: 6996

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding