Tubulin antibody (acLys40)

Catalog No. ABIN7120355

Product Details anti-Tubulin Antibody

Target: Tubulin (TUB)

Binding Specificity: acLys40

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Tubulin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Purification: Protein A+G purification

Purity: ≥95 % as determined by SDS-PAGE

Immunogen: tubulin, alpha 1a

Clone: 6A10

Isotype: IgG1

Application Details

Application Notes: WB: 1:500-1:5000, IF: 1:20-1:200, IHC: 1:20-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide and 50 % glycerol pH 7.3,

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C for 12 months (Avoid repeated freeze / thaw cycles.)

Expiry Date: 12 months

Target Details for Tubulin

Target: Tubulin (TUB)

Alternative Name: tubulin (TUB Products)

Synonyms: 4t antibody, B4t antibody, BETA 98B antibody, CG4869 antibody, DTB1 antibody, Dmbeta4 antibody, Dmel\\CG4869 antibody, T antibody, Tub antibody, beta-Tub antibody, beta-Tub97EF antibody, beta-tub antibody, beta4 antibody, beta4t antibody, beta97F antibody, betaTub4 antibody, betaTub98BC antibody, betaTub98C antibody, beta-Tubulin at 97EF antibody, betaTub97EF antibody

Background: Synonyms:Acetylated alpha tubulin, Acetylated tubulin, acetylated-a-tubulin, acetyl-a-tubulin, alpha tubulin, Alpha tubulin 3, tubulin,TUBA1A Background:Tubulin, composed of heterodimers of alpha and beta tubulin, is the mainly component of microtubules which play important roles in cell motility, mitosis, and intracellular vesicle transport. Both alpha and beta tubulin undergo several posttranslational modifications such as polyglutamylation and acetylation/deacetylation. Tubulin acetylation occurs on lysine-40 at the N-terminal of alpha tubulin and is conserved across species. The histone deacetylase HDAC6 and SIRT2 has been identified as tubulin deacetylases. Reversible acetylation of alpha tubulin may be implicated in regulating microtubule stability, cell motility, and axon regeneration. The level of acetylated tubulin has been linked to epithelial malignancies and sensitivity to chemotherapy. In addition, acetylated tubulin has been widely used as a marker for primary cilia. This antibody is specific to the acetylated tubulin, it does not recognize non-acetylated tubulin.

Molecular Weight: 52 kDa

Gene ID: 7846

UniProt: Q71U36