FOXL1 antibody (AA 104-258)

Catalog No. ABIN7126396

Product Details anti-FOXL1 Antibody

Target: FOXL1 (Forkhead Box L1 (FOXL1))

Binding Specificity: AA 104-258

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FOXL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Immunoprecipitation (IP)

Specificity: FOXL1 is a 337 amino acid protein encoded by the mouse gene FOXL1. FOXL1 belongs to the forkhead family and contains one forkhead DNA-binding domain. The HNF3/forkhead family includes a large number of transcription factors that share a structurally related DNA binding domain. forkhead factors are known to play important roles both during development and in adults. FOXL1 is a winged helix transcriptional regulator expressed in the mesenchymal layer of developing and mature gastrointestinal tract. FOXL1-deficient mice exhibit various defects not only in the epithelial layer of the gastrointestinal tract but also in gut-associated lymphoid tissues. In the small intestine of FOXL1-deficient mice, the formation of Peyer's patches is affected, particularly in the caudal region. FOXL1 is a mesenchymal modifier of the Adenomatous Polyposis Coli (APC) gene products and plays a key role in gastrointestinal tumorigenesis.

Cross-Reactivity (Details): Human. Predicted to react in Rat.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment (around aa104-258) of human FOXL1 protein (exact sequence is proprietary)

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Immunoprecipitation (1-2 μg per 100-500 μg of total protein), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or U87 cells. Ubiquitous tissue expression.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for FOXL1

Target: FOXL1 (Forkhead Box L1 (FOXL1))

Alternative Name: FOXL1 (FOXL1 Products)

Synonyms: zgc:63595 antibody, FOXL1 antibody, fkh6 antibody, fkhl11 antibody, freac7 antibody, CG1132 antibody, Dmel\\CG1132 antibody, Dmfd2 antibody, FD2 antibody, FoxL1 antibody, fd2 antibody, Foxl1 antibody, FKH6 antibody, FKHL11 antibody, FREAC7 antibody, Fkh6 antibody, fkh-6 antibody, forkhead box L1 antibody, forkhead domain 64A antibody, forkhead transcription factor L1 antibody, forkhead box L1 transcription factor antibody, foxl1 antibody, FOXL1 antibody, fd64A antibody, LOC579718 antibody, Foxl1 antibody

Background: FKHL10, FREAC6, JFJ-3, FKH6, FKH11, Forkhead box protein L1, Forkhead related transcription factor, FREAC7,FOXL1 / Forkhead Box Protein L1 (Transcription Factor)
Cellular localisation: Nucleus.

Molecular Weight: 36-41kDa

Gene ID: 2299, 87236

UniProt: Q12951