Recombinant PD-L1 antibody (AA 39-191)
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- Target See all PD-L1 Antibodies
- PD-L1 (CD274 (PD-L1))
- Antibody Type
- Recombinant Antibody
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Binding Specificity
- AA 39-191
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Reactivity
- Human
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Host
- Rabbit
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Clonality
- Monoclonal
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Conjugate
- This PD-L1 antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- PD-L1 is a checkpoint regulator in immune cells, it is expressed on immune or non-hematopoietic cells. Expression of the protein is seen during pregnancy where it has a role in suppressing the immune system. PD-L1 induces an inhibitory signal in activated T-cells and promotes T-cell apoptosis. It is overexpressed in a number of different cancers where it is believed to play a significant role in the cancer s ability to evade the immune system.
- Cross-Reactivity (Details)
- Human and Mouse.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa 39-191) of human CD274 (PD-L1) protein (exact sequence is proprietary)
- Isotype
- IgG
- Top Product
- Discover our top product PD-L1 Primary Antibody
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- Application Notes
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Known_Application: Western Blot (1-2 μg/mL), Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Human heart, placenta, spleen or tonsil tissue. Jurkat, RAW or HepG2 cells.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- PD-L1 (CD274 (PD-L1))
- Alternative Name
- CD274 (PD-L1 Products)
- Synonyms
- B7-H antibody, B7H1 antibody, PD-L1 antibody, PDCD1L1 antibody, PDCD1LG1 antibody, PDL1 antibody, A530045L16Rik antibody, B7h1 antibody, Pdcd1l1 antibody, Pdcd1lg1 antibody, Pdl1 antibody, RGD1566211 antibody, CD274 molecule antibody, CD274 antigen antibody, programmed cell death 1 ligand 2 antibody, CD274 antibody, Cd274 antibody, PDCD1LG2 antibody
- Background
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B7 homolog 1, B7-H1, CD274, PD-L1, PDCD1 ligand 1, PDCD1L1, PDCD1LG1, Programmed cell death 1 ligand 1,PD-L1 / PDCD1LG1 / CD274 / B7-H1 (Cancer Immunotherapy Target)
Cellular localisation: Cell Surface and Cytoplasmic - Molecular Weight
- 37-50kDa
- Gene ID
- 29126, 521989
- UniProt
- Q9NZQ7
- Pathways
- Cancer Immune Checkpoints
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