Recombinant GZMB antibody (AA 73-187)

Catalog No. ABIN7126501

This anti-GZMB antibody is a Rabbit Monoclonal antibody detecting GZMB in IHC and IHC (f). Suitable for Human.

Quick Overview for Recombinant GZMB antibody (AA 73-187) (ABIN7126501)

Target: GZMB (Granzyme B (GZMB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This GZMB antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: GZMB/6530R

Product Details anti-GZMB Antibody

Binding Specificity: AA 73-187

Specificity: Granzyme B is a member of the granule serine protease family stored specifically in NK cells or cytotoxic T cells. Cytolytic T lymphocytes (CTL) and natural killer (NK) cells share the ability to recognize, bind, and lyse specific target cells. They are thought to protect their host by lysing cells bearing on their surface 'nonself' antigens, usually peptides or proteins resulting from infection by intracellular pathogens. Granzyme B is crucial for the rapid induction of target cell apoptosis by CTLs in the cell-mediated immune response. Granzyme B is useful as a marker in the identification of NK/T-cell lymphomas. High percentages of cytotoxic T-cells have been shown to be an unfavorable prognostic indicator in Hodgkin s Disease.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant fragment of human GZMB protein (around aa 73-187) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: A431 cells. Human tonsil, spleen or Hodgkin s Lymphoma tissue (IHC).

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for GZMB

Target: GZMB (Granzyme B (GZMB))

Alternative Name: GZMB

Background: Cathepsin G-like 1, CCPI, CGL1, CSPB, CTLA-1, CTSGL1, Cytotoxic serine protease B, Cytotoxic T lymphocyte associated serine esterase 1, Cytotoxic T-lymphocyte proteinase 2, Fragmentin-2, GRB, Human lymphocyte protein (Hlp), Lymphocyte protease, SECT, T-cell serine protease 1-3E,Granzyme B (NK/T-Cell Lymphoma Marker)
Cellular localisation: Cytoplasmic

Molecular Weight: 29-32kDa

Gene ID: 3002, 1051

UniProt: P10144

Pathways: Apoptosis, Caspase Cascade in Apoptosis