NGFR antibody
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- Target See all NGFR Antibodies
- NGFR (Nerve Growth Factor Receptor (NGFR))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This NGFR antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)), Differentiation (Diff)
- Specificity
- It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGFR is expressed in various neural crest cells and their tumors such as melanocytes, melanomas, neuroblastomas, pheochromocytomas and neurofibromas. Reportedly, anti-NGFR is a reliable marker for desmoplastic and neurotropic melanomas. NGFR is expressed in mature non-neural cells such as perivascular cells, dental pulp cells, lymphoidal follicular dendritic cells, basal epithelium of oral mucosa and hair follicles, prostate basal cells, and myoepithelial cells. Anti-NGFR stains the myoepithelial cells of breast ducts and intra-lobular fibroblasts of breast ducts.
- Cross-Reactivity (Details)
- Human. Expected reactivity in mouse and rat.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- WM245 melanoma cells of human origin.
- Isotype
- IgG1
- Top Product
- Discover our top product NGFR Primary Antibody
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- Application Notes
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Known_Application: Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Neuronal axons, Schwann cells, perineural cells of peripheral nerves, tumors of nerve sheath differentiation. Melanomas.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- NGFR (Nerve Growth Factor Receptor (NGFR))
- Alternative Name
- NGFR (NGFR Products)
- Synonyms
- ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody
- Background
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CD271, Gp80-LNGFR, Low affinity nerve growth factor receptor, Low affinity neurotrophin receptor p75NTR, p75 ICD, p75 Neurotrophin receptor, Tumor necrosis factor receptor superfamily member 16 (TNFRSF16),NGF-Receptor (p75) / CD271 (Soft Tissue Tumor Marker)
Cellular localisation: Cell surface. Cytoplasm. - Molecular Weight
- 75kDa
- Gene ID
- 4804
- UniProt
- P08138
- Pathways
- NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding
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