PKC iota antibody (AA 100-300)
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- Target See all PKC iota (PRKCI) Antibodies
- PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
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Binding Specificity
- AA 100-300
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This PKC iota antibody is un-conjugated
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Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (i±, i²I, i²II and i³) and novel (n) PKC isoforms ( L, , A•, , , l/i, m and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC L and are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.
- Cross-Reactivity (Details)
- Human, Cat and Dog.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant fragment (around aa100-300) of human PRKCI protein (exact sequence is proprietary)
- Isotype
- IgG2b
- Top Product
- Discover our top product PRKCI Primary Antibody
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- Application Notes
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Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Brain, liver or placenta.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))
- Alternative Name
- PRKCI (PRKCI Products)
- Synonyms
- DXS1179E antibody, PKCI antibody, nPKC-iota antibody, aPKC[l] antibody, hal antibody, has antibody, PRKCI antibody, PKC antibody, 2310021H13Rik antibody, AI427505 antibody, PKClambda antibody, Pkci antibody, Pkcl antibody, Prkcl antibody, aPKClambda antibody, mKIAA4165 antibody, protein kinase C iota antibody, protein kinase C, iota antibody, PRKCI antibody, prkci antibody, Prkci antibody
- Background
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APKC lambda/iota, Atypical protein kinase C lambda/iota, Atypical protein kinase C-lambda/iota, nPKC-iota, nPKCiota, PKC lambda/iota, PKCI, PKCiota, PKClambda, PRKC iota, PRKC lambda/iota, PRKCI, Protein kinase C iota type,Protein Kinase C iota / lambda / PRKCI
Cellular localisation: Cytoplasm. Cell Membrane. Nucleus. - Molecular Weight
- 68kDa
- Gene ID
- 5584, 478199
- UniProt
- P41743
- Pathways
- Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation
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