Recombinant Smooth Muscle Actin antibody
-
- Target See all Smooth Muscle Actin (ACTA2) Antibodies
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- Antibody Type
- Recombinant Antibody
-
Reactivity
- Human
-
Host
- Rabbit
-
Clonality
- Monoclonal
-
Conjugate
- This Smooth Muscle Actin antibody is un-conjugated
-
Application
- Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human ACTA2 protein
- Isotype
- IgG
- Top Product
- Discover our top product ACTA2 Primary Antibody
-
-
- Application Notes
-
Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: Blood vessels in all tissues, smooth muscle or leiomyosarcoma.
- Restrictions
- For Research Use only
-
- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
-
- Target
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- Alternative Name
- ACTA2 (ACTA2 Products)
- Synonyms
- AAT6 antibody, ACTSA antibody, MYMY5 antibody, GAMMA-ACTIN antibody, ACT antibody, ACTA3 antibody, ACTE antibody, ACTL3 antibody, ACTSG antibody, Act-4 antibody, Act4 antibody, SMGA antibody, 0610041G09Rik antibody, Actvs antibody, SMalphaA antibody, a-SMA antibody, alphaSMA antibody, wu:fb63d03 antibody, ASMA antibody, SMactin antibody, a-sma antibody, aat6 antibody, actsa antibody, mymy5 antibody, sm-actin antibody, ACTA2 antibody, actc1 antibody, ACT-4 antibody, asma antibody, ACTGE antibody, actin, alpha 2, smooth muscle, aorta antibody, actin, gamma 2, smooth muscle, enteric antibody, actin, alpha 2, smooth muscle, aorta L homeolog antibody, ACTA2 antibody, ACTG2 antibody, Actg2 antibody, Acta2 antibody, acta2 antibody, acta2.L antibody
- Background
-
ACTA2, Actin Alpha 2 Smooth Muscle Aorta, Actin Vascular Smooth Muscle, ACTSA, ACTVS, Alpha Cardiac Actin, Alpha-actin-2, Aortic Smooth Muscle, ASMA, Cell Growth-inhibiting Gene 46 Protein, Growth Inhibiting Gene 46,Actin, Smooth Muscle (Leiomyosarcoma Marker)
Cellular localisation: Cytoplasmic - Molecular Weight
- 42kDa
- Gene ID
- 59, 500483
- UniProt
- P62736
- Pathways
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
-