Recombinant Bcl-2 antibody (AA 41-54)

Catalog No. ABIN7126891

The Mouse Monoclonal anti-Bcl-2 antibody (Clone rBCL2-6418) (ABIN7126891) specifically detects Bcl-2 in WB, IHC, FACS and IF. The antibody is reactive with Human samples.

Quick Overview for Recombinant Bcl-2 antibody (AA 41-54) (ABIN7126891)

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Bcl-2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: RBCL2-6418

Product Details anti-Bcl-2 Antibody

Binding Specificity: AA 41-54

Purpose: Recombinant Bcl-2 (Apoptosis& Follicular Lymphoma Marker) Antibody

Specificity: This antibody recognizes a protein of 25-26 kDa, identified as the bcl-2 alpha oncoprotein. It shows no cross-reaction with Bcl-x or Bax protein. Expression ofbcl-2 alpha oncoprotein inhibits the programmed cell death (apoptosis). In most follicular lymphomas, neoplastic germinal centers express high levels of bcl-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Consequently, this antibody is valuable when distinguishing between reactive and neoplastic follicular proliferation in lymph node biopsies. It may also be used in distinguishing between those follicular lymphomas that express bcl-2 protein and the small number in which the neoplastic cells are bcl-2 negative.

Cross-Reactivity (Details): Human.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: A synthetic peptide, aa41-54 (GAAPAPGIFSSQPG-Cys) of human Bcl-2 protein.

Isotype: IgG2a, kappa

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), ,Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL), ,Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat, K562 or HeLa cells. Humantonsil or follicular lymphomas.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Bcl-2

Target: Bcl-2 (BCL2) (B-Cell CLL/lymphoma 2 (BCL2))

Alternative Name: BCL2

Background:

Apoptosis regulator Bcl-2, B-cell CLL/lymphoma-2

Jurkat, K562 or HeLa cells. tonsil or follicular lymphomas. Skeletal muscle, Spleen, Raji.

Molecular Weight: 25-26kDa

Gene ID: 596, 150749

UniProt: P10415

Pathways: MAPK Signaling, PI3K-Akt Signaling, Apoptosis, Caspase Cascade in Apoptosis, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Skeletal Muscle Fiber Development, Autophagy, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling