RORC antibody
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- Target See all RORC Antibodies
- RORC (RAR-Related Orphan Receptor C (RORC))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This RORC antibody is un-conjugated
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Application
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- This MAb recognizes a protein of 63 kDa, identified as ROR-C. Its epitope maps in between aa1-50. The nuclear orphan receptors RORi± and RORi³ are members of the nuclear hormone receptor superfamily. Members of this family act by directly associating with DNA sequences known as hormone response elements (HREs) and typically bind DNA as either homo- or heterodimers. RORalpha and RORgamma are unique in that they bind DNA as monomers. RORalpha has multiple isoforms that share common DNA and putative ligand-binding domains, but differ in their amino terminal domains, which are generated by alternative RNA processing. RORgamma comprises a 560 amino acid protein that shares 50 % amino acid identity with RORalpha and is most highly expressed in skeletal muscle. Although these proteins are considered orphan receptors, due to a lack of defined ligands, experimental evidence has shown that melatonin may be the natural ligand for these nuclear receptors.
- Cross-Reactivity (Details)
- Human.
- Purification
- 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.
- Immunogen
- Recombinant full-length human RORC protein
- Isotype
- IgG2a
- Top Product
- Discover our top product RORC Primary Antibody
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- Application Notes
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Known_Application: Flow Cytometry (1-2 μg/million cells) (3 % PFA-fixed cells),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
Positive_Control: MOLT4 cells. Human lymphocytes. Human liver or skeletal muscle tissue.
- Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Prepared in 10 mM PBS, WITHOUT BSA and Azide.
- Preservative
- Azide free
- Storage
- -20 °C,-80 °C
- Storage Comment
- Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.
- Expiry Date
- 24 months
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- Target
- RORC (RAR-Related Orphan Receptor C (RORC))
- Alternative Name
- RORC (RORC Products)
- Synonyms
- NR1F3 antibody, RORG antibody, RZR-GAMMA antibody, RZRG antibody, TOR antibody, Nr1f3 antibody, RORgamma antibody, Thor antibody, RAR related orphan receptor C antibody, RAR-related orphan receptor C antibody, RAR-related orphan receptor gamma antibody, RORC antibody, Rorc antibody
- Background
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Nuclear receptor RZR-gamma, Nuclear receptor subfamily 1 group F member 3 (NR1F3), RAR related orphan receptor C, Retinoid-related orphan receptor-gamma (RORG), Rorc, RZR GAMMA, RZRG, TOR,ROR-gamma / RORC (RAR-related Orphan Receptor C)
Cellular localisation: Nuclear - Molecular Weight
- 63kDa
- Gene ID
- 6097
- UniProt
- P51449
- Pathways
- Nuclear Receptor Transcription Pathway, Steroid Hormone Mediated Signaling Pathway
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