Recombinant SDHB antibody

Catalog No. ABIN7126928

This anti-SDHB antibody is a Rabbit Monoclonal antibody detecting SDHB in IHC (f). Suitable for Human.

Quick Overview for Recombinant SDHB antibody (ABIN7126928)

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This SDHB antibody is un-conjugated

Application: Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Product Details anti-SDHB Antibody

Specificity: Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane. Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Synthetic peptide corresponding to residues in human SDHB was used as an immunogen.

Isotype: IgG

Application Details

Application Notes:

Known_Application: Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Jurkat or HepG2 cells. Human kidney or heart tissue.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for SDHB

Target: SDHB (Succinate Dehydrogenase Complex, Subunit B, Iron Sulfur (Ip) (SDHB))

Alternative Name: SDHB

Background: CWS2, DHSB, Iron-sulfur subunit of complex II, PGL4, SDH2, SDHB, SDHIP, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, Succinate Dehydrogenase Complex Subunit B Iron Sulfur Protein,SDHB (Succinate Dehydrogenase B) (Pheochromocytoma Marker)
Cellular localisation: Cytoplasm. Mitochondria.

Molecular Weight: 32kDa

Gene ID: 6390, 465924

UniProt: P21912