Recombinant Vimentin antibody (AA 2-466)

Catalog No. ABIN7127048

The Mouse Monoclonal anti-Vimentin antibody (Clone rVIM-6431) (ABIN7127048) specifically detects Vimentin in WB and IHC. The antibody is reactive with Human, Rat, Pig, Cow, Chicken, Dog, Cat and Horse samples.

Quick Overview for Recombinant Vimentin antibody (AA 2-466) (ABIN7127048)

Target: Vimentin (VIM)

Antibody Type: Recombinant Antibody

Reactivity: Human, Rat, Pig, Cow, Chicken, Dog, Cat, Horse

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RVIM-6431

Product Details anti-Vimentin Antibody

Binding Specificity: AA 2-466

Purpose: Recombinant Vimentin (Mesenchymal Cell Marker) Antibody

Specificity: This MAb reacts with a 58 kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP s) such as desmin, keratin, neurofilament, and glial fibrillary acid protein.Anti-vimentin alone is of limited value as a diagnostic tool, however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

Cross-Reactivity (Details): Human, Rat, Horse, Chicken, Cow, Cat, Dog, and Pig. Very broad species reactivity. Does not react with Mouse.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes:

Known_Application: Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: U-87, Raji, Jurkat or HeLa cells. Human lymph node or tonsil.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: VIM

Background:

VIM

Raji, U-87, Jurkat or HeLa cells. Human lymph node or tonsil. Human Heart, Human Skeletal muscle, Human Skin, A549.

Molecular Weight: 57-60kDa

Gene ID: 7431, 455493

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis