NMI antibody

Catalog No. ABIN7127126

Product Details anti-NMI Antibody

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This NMI antibody is un-conjugated

Application: Flow Cytometry (FACS)

Specificity: Nmi (for N-Myc interactor) is an interferon inducible protein that associates with multiple transcription factors, including c-Myc, n-Myc, Max, and c-Fos, which contain bHLH-ZIP, bHLH, or Zip domains. Nmi is ubiquitously expressed at low levels throughout various fetal and adult tissues and at higher levels in myeloid leukemias and cell lines overexpressing c-Myc. In addition to binding Myc proteins, Nmi also associates with the Stat family of transcription factors, where it enhances Stat-dependent transcription. Although Nmi lacks an intrinsic DNA binding or activation domain, Nmi enhances the transcriptional activity of the Stat proteins, in response to cytokine stimulation, by recruiting the Stat1 and Stat5 transcriptional coactivators, CREB-binding protein (CBP) and p300. In vitro studies indicate that Nmi, expressed in conjunction with CBP, enhances the transcriptional responsiveness of Stat5 to IL-2 stimulation five-fold over CBP alone by increasing the affinity of Stat proteins for CBP/p300.

Cross-Reactivity (Details): Human

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Recombinant full-length human NMI protein

Isotype: IgG2b

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells),Optimal dilution for a specific application should be determined.

Positive_Control: HeLa or K562 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for NMI

Target: NMI (N-Myc (And STAT) Interactor (NMI))

Alternative Name: NMI (NMI Products)

Synonyms: fj02e01 antibody, wu:fj02e01 antibody, NMI antibody, MGC108489 antibody, N-myc and STAT interactor antibody, N-myc (and STAT) interactor antibody, N-myc and STAT interactor L homeolog antibody, NMI antibody, Nmi antibody, nmi antibody, nmi.L antibody

Background: N myc (and STAT) interactor, N myc interactor, NMI, NMYC and STAT interactor, NMYC interactor,N myc (and STAT) Interactor / NMI
Cellular localisation: Nucleus.

Molecular Weight: 38kDa

Gene ID: 9111, 54483

UniProt: Q13287