CD151 antibody

Catalog No. ABIN7127232

Product Details anti-CD151 Antibody

Target: CD151

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD151 antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Specificity: CD151 is involved in a wide variety of cell biological processes, including cell adhesion and the transport of integrins via vesicles. The human CD151 gene maps to chromosome 11p15.5 and encodes a 253 amino acid protein, which belongs to the tetraspan (4TM) superfamily. CD151 can associate with several integrin chains including 1, 3, 4, i±2, i±3, i±5 and i±6 integrins. CD151 may provide a framework for the spatial organization of both type I and type II hemidesmosomes, which are specialized junctional complexes that function as cell attachment sites for binding to basement membranes. CD151 RNA transcript (1.6 kb) can be detected in MO7e cells, bone marrow stromal cells, C11 endothelial cells, HUVEC and several myeloid leukemia cell lines, however, no transcript is detected in brain and the lymphoblastoid cell lines MOLT-4 and BALM-1. Leu 149-Glu 213 of CD151 is the interface through which Integrin i±3/ 1 can bind. CD151 can enhance cell motility, invasion and metastasis of cancer cells in a focal adhesion kinase dependent manner.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: The details of the immunogen for this antibody are not available.

Isotype: IgG1

Application Details

Application Notes:

Known_Application: Flow Cytometry (1-2 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (1-2 μg/mL),Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.

Positive_Control: Human tonsil or spleen. HT1080 cells.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for CD151

Target: CD151

Alternative Name: CD151 (CD151 Products)

Synonyms: CD151 antibody, MGC83668 antibody, gp27 antibody, mer2 antibody, raph antibody, sfa1 antibody, peta-3 antibody, tspan24 antibody, PETA-3 antibody, SFA-1 antibody, Tspan24 antibody, GP27 antibody, MER2 antibody, RAPH antibody, SFA1 antibody, TSPAN24 antibody, CD151 molecule antibody, CD151 molecule (Raph blood group) L homeolog antibody, CD151 molecule (Raph blood group) antibody, CD151 antigen antibody, CD151 antibody, cd151.L antibody, cd151 antibody, Cd151 antibody

Background: GP27, hemidesmosomal tetraspanin CD151, Membrane glycoprotein SFA-1, MER2, Platelet endothelial tetraspan antigen 3 (PETA3), platelet surface glycoprotein gp27, Platelet-endothelial cell tetraspan antigen 3, Platelet-endothelial tetraspan antigen 3, RAPH, Red blood cell antigen MER2, SFA1, Tetraspanin-24 (TSPAN24),CD151
Cellular localisation: Cell Surface

Molecular Weight: 28-32kDa

Gene ID: 977, 654379

UniProt: P48509