ITGAV/ITGB6 antibody

Catalog No. ABIN7127264

Product Details anti-ITGAV/ITGB6 Antibody

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGAV/ITGB6 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), ELISA, Immunoprecipitation (IP), Coating (Coat), Immunofluorescence (IF)

Specificity: Integrins are heterodimers composed of noncovalently associated transmembrane i± and i² subunits. The 16 i± and 8 i² subunits heterodimerize to produce more than 20 different receptors. Most integrin receptors bind ligands that are components of the extracellular matrix, including Fibronectin, Collagen and Vitronectin. Certain integrins can also bind to soluble ligands such as Fibrinogen, or to counterreceptors on adjacent cells such as the intracellular adhesion molecules (ICAMs), leading to aggregation of cells. Ligands serve to cross-link or cluster integrins by binding to adjacent integrin receptors, both receptor clustering and ligand occupancy are necessary for the activation of integrin-mediated responses. In addition to mediating cell adhesion and cytoskeletal organization, integrins function as signaling receptors. Signals transduced by integrins play a role in many biological processes, including cell growth, differentiation, migration and apoptosis.

Cross-Reactivity (Details): Human.

Purification: 1.0mg/ml of Ab purified from Bioreactor by Protein A/G.

Immunogen: Mouse fibroblasts retrovirally infected with cDNA to human beta6, Cells expressed a mouse/human avb6 chimera.

Isotype: IgG2a

Application Details

Application Notes:

Known_Application: ELISA (For coating use Ab at 1-5 μg/mL, order Ab without BSA), Immunoprecipitation (1-2 μg/500 μg protein), ,Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1 μg/mL),Optimal dilution for a specific application should be determined.

Positive_Control: Squamous cell carcinoma cells. A375M melanoma cell line. A549 cell line.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10 mM PBS, WITHOUT BSA and Azide.

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous.

Expiry Date: 24 months

Target Details for ITGAV/ITGB6

Target: ITGAV/ITGB6 (Integrin AlphaV Beta6 (ITGAV/ITGB6))

Alternative Name: ITGAV & ITGB6 (ITGAV/ITGB6 Products)

Background: Antigen identified by monoclonal antibody L230, CD 51, CD51, Integrin alpha five, integrin alpha V beta 3, Integrin alpha-V light chain, integrin alphaVbeta3, integrin, alpha V (vitronectin receptor, alpha polypeptide, antigen CD51), ITGAV, MSK 8, Msk8, Vitronectin receptor subunit alpha, VNRA, VTNR, ITGB6, Integrin, beta 6,Integrin alpha V + beta 6
Cellular localisation: Membrane.

Molecular Weight: 150-160kDa under non-reducing conditions. Under reducing conditions, cleaved into 2 bands of ~125-130kDa +20-25kDa

Gene ID: 3685, 3694, 436873

UniProt: P06756, P18564