NFKBIB antibody (pSer23)

Catalog No. ABIN7138495

Product Details anti-NFKBIB Antibody

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Binding Specificity: pSer23

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NFKBIB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Cross-Reactivity: Human, Mouse, Rat

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of serine 23 (L-G-S(p)-L-G) derived from Human IkB-b.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000, IHC:1:50-1:100,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied at 1.0 mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for NFKBIB

Target: NFKBIB (NF-kappa-B inhibitor beta (NFKBIB))

Alternative Name: NFKBIB (NFKBIB Products)

Synonyms: NFKBIB antibody, IKB-beta antibody, IKappaBbeta antibody, IkB antibody, IkBb antibody, IKBB antibody, TRIP9 antibody, NFKB inhibitor beta antibody, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta antibody, NFKBIB antibody, Nfkbib antibody

Background:

Background:

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further IKBA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.

Shirane, M. et al. (1999) J Biol Chem 274, 28169-28174.
DiDonato J, et al. (1996) Mol Cell Biol 16(4): 1295-304

Aliases: I kappa B beta antibody, I-kappa-B-beta antibody, IkappaBbeta antibody, IKB beta antibody, IkB-B antibody, IkB-beta antibody, IKBB antibody, IKBB_HUMAN antibody, IkBbeta antibody, NF kappa BIB antibody, NF-kappa-B inhibitor beta antibody, NF-kappa-BIB antibody, Nfkbib antibody, Thyroid receptor interacting protein 9 antibody, Thyroid receptor-interacting protein 9 antibody, TR interacting protein 9 antibody, TR-interacting protein 9 antibody, TRIP-9 antibody, TRIP9 antibody

UniProt: Q15653

Pathways: NF-kappaB Signaling, Activation of Innate immune Response, Maintenance of Protein Location, Toll-Like Receptors Cascades