Insulin Receptor antibody (pTyr1355)

Catalog No. ABIN7138935

The Rabbit Polyclonal anti-Insulin Receptor antibody has been validated for WB and ELISA. It is suitable to detect Insulin Receptor in samples from Human.

Quick Overview for Insulin Receptor antibody (pTyr1355) (ABIN7138935)

Target: Insulin Receptor (INSR)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Insulin Receptor antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Insulin Receptor Antibody

Binding Specificity: pTyr1355

Cross-Reactivity: Human

Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Immunogen: Peptide sequence around phosphorylation site of tyrosine 1355 (R-S-Y(p)-E-E) derived from Human IR.

Isotype: IgG

Application Details

Application Notes: WB:1:500-1:1000,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for Insulin Receptor

Target: Insulin Receptor (INSR)

Alternative Name: INSR

Background:

Background:

Receptor tyrosine kinase which mediates the pleiotropic actions of insulin. Binding of insulin leads to phosphorylation of several intracellular substrates, including, insulin receptor substrates (IRS1, 2, 3, 4), SHC, GAB1, CBL and other signaling intermediates. Each of these phosphorylated proteins serve as docking proteins for other signaling proteins that contain Src-homology-2 domains (SH2 domain) that specifically recognize different phosphotyrosines residues, including the p85 regulatory subunit of PI3K and SHP2.

Tennagels N, et al. (2001) Biochem Biophys Res Commun 282, 387-93
Tennagels N, Bergschneider E, Al-Hasani H, Klein HW (2000) FEBS Lett 479, 67-71
Noelle V, Tennagels N, Klein HW (2000) Biochemistry 39, 7170-7

Aliases: CD220 antibody, HHF5 antibody, human insulin receptor antibody, Insr antibody, INSR_HUMAN antibody, Insulin receptor subunit beta antibody, IR 1 antibody, IR antibody, IR-1 antibody, IR1 antibody

UniProt: P06213

Pathways: NF-kappaB Signaling, RTK Signaling, AMPK Signaling, Carbohydrate Homeostasis, Regulation of Cell Size, Regulation of Carbohydrate Metabolic Process, Growth Factor Binding, Negative Regulation of Transporter Activity