TDG antibody (AA 141-410)

Catalog No. ABIN7153379

This Rabbit Polyclonal antibody specifically detects TDG in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for TDG antibody (AA 141-410) (ABIN7153379)

Target: TDG (Thymine-DNA Glycosylase (TDG))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TDG antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TDG Antibody

Binding Specificity: AA 141-410

Cross-Reactivity: Human, Mouse

Purification: Antigen Affinity Purified

Immunogen: Recombinant Human G/T mismatch-specific thymine DNA glycosylase protein (141-410AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS with 0.02 % sodium azide, 50 % glycerol, pH 7.3.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for TDG

Target: TDG (Thymine-DNA Glycosylase (TDG))

Alternative Name: TDG

Background:

Background: DNA glycosylase that plays a key role in active DNA demethylation: specifically recognizes and binds 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) in the context of CpG sites and mediates their excision through base-excision repair (BER) to install an unmethylated cytosine. Cannot remove 5-hydroxymethylcytosine (5hmC). According to an alternative model, involved in DNA demethylation by mediating DNA glycolase activity toward 5-hydroxymethyluracil (5hmU) produced by deamination of 5hmC. Also involved in DNA repair by acting as a thymine-DNA glycosylase that mediates correction of G/T mispairs to G/C pairs: in the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. Its role in the repair of canonical base damage is however minor compared to its role in DNA demethylation. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine.

Aliases: C JUN leucine zipper interactive protein antibody, C JUN leucine zipper interactive protein antibody, C-JUN leucine zipper interactive protein JZA-3 antibody, E130317C12Rik antibody, EC 3.2.2.29 antibody, G/T mismatch specific thymine DNA glycosylase antibody, G/T mismatch specific thymine DNA glycosylase antibody, G/T mismatch-specific thymine DNA glycosylase antibody, JZA 3 antibody, Jza1 antibody, T:G mismatch thymine glycosylase antibody, Tdg antibody, TDG_HUMAN antibody, Thymine DNA glycosylase antibody, Thymine-DNA glycosylase antibody

UniProt: Q13569

Pathways: DNA Damage Repair, Chromatin Binding