PCNA antibody (AA 1-261)

Catalog No. ABIN7164738

The Rabbit Polyclonal anti-PCNA antibody has been validated for WB, IHC, ELISA and IF. It is suitable to detect PCNA in samples from Human.

Quick Overview for PCNA antibody (AA 1-261) (ABIN7164738)

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PCNA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PCNA Antibody

Binding Specificity: AA 1-261

Cross-Reactivity: Human, Mouse

Purification: >95%, Protein G purified

Immunogen: Recombinant Human Proliferating cell nuclear antigen protein (1-261AA)

Isotype: IgG

Application Details

Application Notes: Recommended dilution: WB:1:2000-1:10000, IHC:1:20-1:200, IF:1:50-1:200,

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Preservative: 0.03 % Proclin 300
Constituents: 50 % Glycerol, 0.01M PBS, PH 7.4

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C,-80 °C

Storage Comment: Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Target Details for PCNA

Target: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Alternative Name: PCNA

Background:

Background: Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase\'s processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3\'-5\' exonuclease and 3\'-phosphodiesterase, but not apurinic-apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while \'Lys-63\'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.

Aliases: ATLD2 antibody, cb16 antibody, Cyclin antibody, DNA polymerase delta auxiliary protein antibody, etID36690.10 antibody, fa28e03 antibody, fb36g03 antibody, HGCN8729 antibody, MGC8367 antibody, Mutagen-sensitive 209 protein antibody, OTTHUMP00000030189 antibody, OTTHUMP00000030190 antibody, PCNA antibody, Pcna/cyclin antibody, PCNA_HUMAN antibody, PCNAR antibody, Polymerase delta accessory protein antibody, Proliferating cell nuclear antigen antibody, wu:fa28e03 antibody, wu:fb36g03 antibody

UniProt: P12004

Pathways: Telomere Maintenance, DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy